LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2156..2252) /label=attL2 /note="attL2 (c)" misc_signal complement(2267..2286) /note="T7 Promoter/priming site (c)" primer_bind 2294..2310 /note="M13 Reverse priming site" gene 2423..3232 /note="Kanamycin resistance gene" rep_origin 3353..4026 /note="pUC origin" PCR_frame 669..2156 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2176) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2173) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2164..4029,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2134..2154 /note="TEV site" source 673..2133 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:119426 IMAGE:40007344" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: AACTCAT sequenced from the forward primer" gene 673..2133 /gene="UGT3A2" /db_xref="GeneID:167127" /db_xref="HGNC:27266" CDS 673..2133 /dnas_title="UDP glycosyltransferase 3 family, polypeptide A2" /gene="UGT3A2" /codon_start=1 /product="UDP glycosyltransferase 3 family, polypeptide A2" /protein_id="AAI03925.1" /db_xref="GI:74355614" /db_xref="GeneID:167127" /db_xref="HGNC:27266" /translation="MAGQRVLLLVGFLLPGVLLSEAAKILTISTVGGSHYLLMDRVSQ ILQDHGHNVTMLNHKRGPFMPDFKKEEKSYQVISWLAPEDHQREFKKSFDFFLEETLG GRGKFENLLNVLEYLALQCSHFLNRKDIMDSLKNENFDMVIVETFDYCPFLIAEKLGK PFVAILSTSFGSLEFGLPIPLSYVPVFRSLLTDHMDFWGRVKNFLMFFSFCRRQQHMQ STFDNTIKEHFTEGSRPVLSHLLLKAELWFINSDFAFDFARPLLPNTVYVGGLMEKPI KPVPQDLENFIAKFGDSGFVLVTLGSMVNTCQNPEIFKEMNNAFAHLPQGVIWKCQCS HWPKDVHLAANVKIVDWLPQSDLLAHPSIRLFVTHGGQNSIMEAIQHGVPMVGIPLFG DQPENMVRVEAKKFGVSIQLKKLKAETLALKMKQIMEDKRYKSAAVAASVILRSHPLS PTQRLVGWIDHVLQTGGATHLKPYVFQQPWHEQYLLDVFVFLLGLTLGTLWLCGKLLG MAVWWLRGARKVKET" misc_feature 673..2133 /note="UGT3A2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctgg gcagcgagtg cttcttctag tgggcttcct tctccctggg 721 gtcctgctct cagaggctgc caaaatcctg acaatatcta cagtaggtgg aagccattat 781 ctactgatgg accgggtttc tcagattctt caagatcacg gtcataatgt caccatgctt 841 aaccacaaaa gaggtccttt tatgccagat tttaaaaagg aagaaaaatc atatcaagtt 901 atcagttggc ttgcacctga agatcatcaa agagaattta aaaagagttt tgatttcttt 961 ctggaagaaa ctttaggtgg cagaggaaaa tttgaaaact tattaaatgt tctagaatac 1021 ttggcgttgc agtgcagtca ttttttaaat agaaaggata tcatggattc cttaaagaat 1081 gagaacttcg acatggtgat agttgaaact tttgactact gtcctttcct gattgctgag 1141 aagcttggga agccatttgt ggccattctt tccacttcat tcggctcttt ggaatttggg 1201 ctaccaatcc ccttgtctta tgttccagta ttccgttcct tgctgactga tcacatggac 1261 ttctggggcc gagtgaagaa ttttctgatg ttctttagtt tctgcaggag gcaacagcac 1321 atgcagtcta catttgacaa caccatcaag gaacatttca cagaaggctc taggccagtt 1381 ttgtctcatc ttctactgaa agcagagttg tggttcatta actctgactt tgcctttgat 1441 tttgctcgac ctctgcttcc caacactgtt tatgttggag gcttgatgga aaaacctatt 1501 aaaccagtac cacaagactt ggagaacttc attgccaagt ttggggactc tggttttgtc 1561 cttgtgacct tgggctccat ggtgaacacc tgtcagaatc cggaaatctt caaggagatg 1621 aacaatgcct ttgctcacct accccaaggg gtgatatgga agtgtcagtg ttctcattgg 1681 cccaaagatg tccacctggc tgcaaatgtg aaaattgtgg actggcttcc tcagagtgac 1741 ctcctggctc acccaagcat ccgtctgttt gtcacccacg gcgggcagaa tagcataatg 1801 gaggccatcc agcatggtgt gcccatggtg gggatccctc tctttggaga ccagcctgaa 1861 aacatggtcc gagtagaagc caaaaagttt ggtgtttcta ttcagttaaa gaagctcaag 1921 gcagagacat tggctcttaa gatgaaacaa atcatggaag acaagagata caagtccgcg 1981 gcagtggctg ccagtgtcat cctgcgctcc cacccgctca gccccacaca gcggctggtg 2041 ggctggattg accacgtcct ccagacaggg ggcgcgacgc acctcaagcc ctatgtcttt 2101 cagcagccct ggcatgagca gtacctgctc gacgaaaact tgtactttca aggctCCAGC 2161 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2221 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2281 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2341 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2401 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2461 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2521 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2581 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2641 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2701 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2761 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2821 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2881 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2941 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3001 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3061 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3121 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3181 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3241 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3301 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3361 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3421 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3481 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3541 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3601 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3661 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3721 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3781 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3841 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3901 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3961 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4021 TCACATGTT //